Composite
Part:BBa_K1392971:Design
Designed by: Şeniz Yüksel İlkem Kumru Group: iGEM14_METU_Turkey (2014-10-10)
Holin+Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No design considerations during Holin Gene
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 9 residues.
Source
Brown iGEM Team by Ry Young at Texas A&M Modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs